Contact Person

Dr. Peter Henning

Managing Editor

Phone: +49 (0)711 - 2 29 87 37
Fax: +49 (0)711 - 2 29 87 65
send an Email

Archive (2015–2005)

Multiplicity Issues in Microarray Experiments

Journal: Methods of Information in Medicine
Subtitle: A journal stressing, for more than 50 years, the methodology and scientific fundamentals of organizing, representing and analyzing data, information and knowledge in biomedicine and health care
ISSN: 0026-1270
Issue: 2005 (Vol. 44): Issue 3 2005
Pages: 431-437

Multiplicity Issues in Microarray Experiments

F. Bretz1 , J. Landgrebe2 , E. Brunner3

1 B&SR, Novartis Pharma AG, Basel, Switzerland 2 Abteilung Biochemie II, Universität Göttingen, Göttingen, Germany 3 Abteilung Medizinische Statistik, Universität Göttingen, Göttingen, Germany


Screening, Familywise error rate, false discovery rate, error concepts


Objectives: Discussion of different error concepts relevant to microarray experiments. Review of some commonly used multiple testing procedures. Comparison of different approaches as applied to gene expression data. Methods: This article focuses on familywise error rate (FWER) and false discovery rate (FDR) controlling procedures. Methods under investigation include: Bonferroni-type methods and their improvements (including resampling approaches), modified Bonferroni methods, data-driven approaches, as well as the linear step-up method and its modifications. Particular emphasis lies on the description of the assumptions, advantages and limitations for the investigated methods. Results: FWER controlling procedures are often too conservative in high dimensional screening studies. A better balance between the raw P -values and the stringent FWER-adjusted P -values may be required in many situations, as provided by FDR controlling and related procedures. Conclusions: The questions remain open, which error concept to apply and which multiple testing procedure to use. Although we believe that the FDR or one of its variants will be applied more often in the future, longterm experience with microarray technology is missing and thus the validity of appropriate multiple test procedures cannot yet be assessed for microarray data analysis.

You may also be interested in...

Manuel Monreal 1, Luis Peidro 2, Carlos Resines 3, Carlos Garcés 4, José Luís Fernández 5, Eduardo Garagorri 6, Juan Carlos González 7, for the NETCOT Investigators*

Thromb Haemost 2008 99 6: 1112-1115


M. Dietlein 1, H. Wieler 2, M. Schmidt 1, R. Schwab 3, P. E. Goretzki 4, H. Schicha1

Nuklearmedizin 2008 47 2: 65-72

Falk Thielemann, Jörg Ziegler, Klaus Peter Günther

Kinder- und Jugendmedizin 2007 7 7: 371-378

Methods issue 5/2015

At last year's annual conference of the German Association for Medical Informatics, Biometry and...

Methods issue 4/2015

Elske Ammenwerth submitted the paper "Evidence-based Health Informatics: How Do We Know What...

Methods issue 3/2015

The focus theme in this issue on "Biosignal Interpretation II – Advanced Methods for Studying...

Methods issue 2/2015

Over the past few years there has been an increasing interest in the application of Virtual Reality...